Reliable CD8 antibody for Flow Cytometry

June 05, 2018

BlueskyReddit
GIVI
The Gladstone Institutes
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BV605 Mouse Anti-Human CD8

Catalog Number:

564116

My lab investigates markers for detecting the latent HIV reservoir. Specifically, we look at tissue resident memory CD4 T-cells. We need a antibody to reliably gate out CD8+ T-cells and this product from BD has been perfect to achieve that.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human Colonoscopies

Primary Incubation

1:1000 at 4 C for 30 min

Blocking Agent

1:1000 FC block at 4C for 30 min

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Results clearly separate the CD8 population from the rest of the CD3+ cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Provides consistent results

The Bad

Nothing

The Bottom Line

A great product from BD for flow cytometry!

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