Great CD3 Antibody for Flow Cytometry

GIVI
The Gladstone Institutes
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PerCP-Cy™5.5 Mouse Anti-Human CD3-C

Catalog Number:

560835

My lab investigates markers for identifying the latent HIV reservoir. As such, we need a reliable marker of T-cells. This CD3 percpcy5.5 antibody from BD Biosciences has proved to do a terrific job in distinguishing these T-lymphocytes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human Colonoscopies

Primary Incubation

1:300 at 4C for 30 min

Blocking Agent

1:1000 FC block at 4C for 30 min

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Results clearly distinguish CD3+ populations amongst the rest. We can use this to gate for further investigation.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use and reliable

The Bad

A bit expensive

The Bottom Line

Great antibody for flow cytometry!

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