Flow Cytometry Marker For Long Term Hematopoietic Stem Cells

May 08, 2018

BlueskyReddit
Pharmaceutical Technology
CIBER BBN
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Company:

Biolegend

Product Name:

APC anti-mouse CD150 (SLAM) Antibody

Catalog Number:

115909

We used this marker in the identification and isolation of murine long term hematopoietic stem cells from bone marrow. This antibody labels CD150 with a good affinity.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine bone marrow

Primary Incubation

1:100

Blocking Agent

anti-CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

In APC channel

Results Summary

We identify with this marker in combination with CD48 and CD41 the long term hematopietic stem cells from bone marrow gated Sca+ cKit+ lineage - alive cells. We can analyze and sort these cells with the flow cytometer.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good performance

The Bad

None

The Bottom Line

Good price

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