Strong CD56 Signal

Toxicology
University of Kentucky
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

CD56 PE

Catalog Number:

340724

We do a lot of NK cell staining. We needed a good CD56 antibody that gives us a good resolution of CD56(dim) from CD56(bright) and also from CD56(negative). We chose this antibody because PE is supposed to be a bright fluorochrome.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human white blood cells

Primary Incubation

5uL at 4C for 15 minutes

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometer (FL-2)

Results Summary

The results show the NK cells clearly separated from other leukocytes. We can see a small CD56(bright) population. It was easy to gate on NK cells (purple).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good resolution. Bright signal

The Bad

A bit expensive

The Bottom Line

A good antibody to use for NK cell staining!

Share your experience with other scientists. Write a Review! »

Join the discussion