CD32 PE flow antibody to stain FcgammaRII

Pediatrics
Emory University School of Medicine
Post Doctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Caprico Biotechnologies

Product Name:

Anti-human CD32 PE

Catalog Number:

102725

This antibody was used to determine FcγRII surface expression in human CD14 monocytes after blocking them with anti-CD32. The unblocked human CD14 monocytes showed high expression of FcγRII. The antibody needs to be titrated a lot and still gives a distinct positive separation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human monocytes

Primary Incubation

30 minutes at 4C

Blocking Agent

FACS buffer containing 2% FBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

The product worked well, staining for FcγRII.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Antibody works a very low concentration

The Bad

None

The Bottom Line

I would recommend using the antibody.

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