Cell Signaling Technology
beta-Catenin (D10A8) XP Rabbit mAb
8480
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We have identified a class of ATPase inhibitors that show promise for treatment of ovarian cancer. We are currently evaluating what mechanisms, if any, may lead to resistance to theses ATPase inhibitors. We are measuring changes in protein expression in an animal model of drug resistance. I chose this product because of CST's reputation for reliable antibodies.
Western Blot
Cellular ascites from PDX mouse models of resistance (lysates)
1:1000 dilution in 5% milk in TBS-T, overnight at 4 degrees C
5% milk in TBS-T
1:10,000 in 5% milk in TBS-T, 30 minutes at RT
N/A
Standard ECL solution (Pierce), 1 minute incubation
Single band on western blot, which is slightly curious since in my experience pan beta-catenin antibodies pick up the numerous phospho/dephospho beta-catenin proteins.
Clean blot...
Does not pick up expected posttranslationally modified protein
I would not use this antibody to investigate projects related to bcat. Wnt signaling pathway activity.