Cell Signaling Technology
Claudin-1 (D5H1D) XP Rabbit mAb
13255
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We have identified a class of ATPase inhibitors that show promise for treatment of ovarian cancer. We are currently evaluating what mechanisms, if any, may lead to resistance to theses ATPase inhibitors. We are measuring changes in protein expression in an animal model of drug resistance. I chose this product because of CST's reputation for reliable antibodies.
Western Blot
Cellular ascites from PDX mouse models of resistance (lysates)
1:1000 dilution in 5% milk in TBS-T, overnight at 4 degrees C
5% milk in TBS-T
1:10,000 in 5% milk in TBS-T, 30 minutes at RT
N/A
Standard ECL solution (Pierce), 1 minute incubation
Very, very nice bands. Antibody worked on the first try, with no optimization needed.
Great bands on first try
No problems with antibody!
CST has shown they make and sell reliable, good quality antibodies. This antibody doesn't disappoint.