Best Stress Granule Antibody

Neuroscience
Thomas Jefferson University
Postdoctoral Fellow

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Company:

Proteintech

Product Name:

G3BP1 antibody

Catalog Number:

13057-2-AP

My study aims to understand how stress granules are linked to amyotrophic lateral sclerosis pathology. Specifically to understand the dynamics of stress granule assembly and disassembly in neuronal cells. The marker of stress granules, G3BP1 antibody, from Proteintech gives a highly specific immunofluorescence signal. We have validated this in our paper.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Cultured rat neurons

Primary Incubation

1:500 overnight at 4 degrees in PBS with 2% blocking solution

Blocking Agent

10% horse serum plus 2% BSA

Secondary Incubation

1:500 Alexa Fluor 1 hour at room temperature in dark

Tertiary Incubation

Not applicable

Detection

Confocal microscopy

Results Summary

Upon induction of sodium arsenite stress in neuron G3BP1 positive stress granules formed in 90 minutes.

DOI or PMID #

26728149

Additional Notes

Not applicable

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Summary

The Good

Highly specific

The Bad

None

The Bottom Line

Useful tool for stress granule studies

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