Cleaved Caspase-3 Antibody

April 16, 2018

Center for Translational Biomedical Research
UNC Greensboro
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Company:

Cell signaling technology

Product Name:

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb

Catalog Number:

9579

This antibody was used in a study that investigated the role of ALOX15 and its product 13-HODE in alcoholic fatty liver. It has been published in Sci Rep. in 2017. The antibody reviewed here gave best results in western blot using mouse liver.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse liver

Primary Incubation

1:1000, 10 hour at 4 degree

Blocking Agent

5% milk

Secondary Incubation

1:5000, 1 hour at 25 degree

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Alcohol feeding significantly increased the expression of cleaved caspase 3, which was suppressed by ALOX15 knockout.

DOI or PMID #

28827690

Additional Notes

N/A

Image Gallery

Summary

The Good

Very specific

The Bad

Signal is kind of weak

The Bottom Line

Good for western blot

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