Easy To Use Mouse CD45.2 Cell Marker

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC anti-mouse CD45.2 Antibody

Catalog Number:

109806

In our lab we study the proliferation of transferred T cells in the mouse model. To do so, we use CD45.2 recipient mice and CD45.1 donor mice. We need an antibody that works well to stain only the recipient mouse cells to differentiate them from the donor mouse cells. This antibody works well to do this.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung cells

Primary Incubation

Cells were stained in 100uL FACS buffer with antibody at 1:200; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

Using this antibody, we can successfully identify specifically CD45.2 cells extracted from our mouse models.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Not too bright, good price

The Bad

None

The Bottom Line

This antibody works well for cells from mouse tissue.

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