Antibody For Hematopoietic Stem Cells

Pharmaceutical Technology
CIBER BBN
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Company:

Biolegend

Product Name:

PE anti-mouse Ly-6A/E (Sca-1) Antibody

Catalog Number:

122507

This antibody can be used for the identification of hematopoietic stem cells isolated from the bone marrow. We use this antibody because of its good performance and low price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Bone marrow from mice

Primary Incubation

1:100

Blocking Agent

Purified anti-CD16/32 (2.4G2)

Secondary Incubation

None

Tertiary Incubation

None

Detection

PE channel

Results Summary

From alive and lineage negative gated cells, HSCs are positive stained with Sca, that in combination with cKit antibody helps to identify the HSCs population. This cells can be sorted through flow cytometry sorting.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good performance

The Bad

None

The Bottom Line

Good price for purchasing.

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