Hematopoietic Stem Cells Identification

May 10, 2018

Pharmaceutical Technology
CIBER BBN
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Company:

Biolegend

Product Name:

APC anti-mouse CD117 (c-Kit) Antibod

Catalog Number:

105811

All hematopoietic stem cells (HSCs) are c-Kit positive and this antibody can be used to identified the aforementioned cell population. It has a reasonable price and it stains HSCs very well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Bone marrow from mice

Primary Incubation

1:100

Blocking Agent

Purified anti-CD16/32 (2.4G2)

Secondary Incubation

None

Tertiary Incubation

None

Detection

APC channel

Results Summary

We used this antibody to identify hematopoietic stem cells. First we gated alive and lineage negative cells and next we stained with Sca and cKit since HSCs are positive for these stains.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Reasonable price and good performance

The Bad

None

The Bottom Line

I recommend to purchase it.

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