Time Course Effect Of Chemoattractant On VCAM1 Expression

April 19, 2018

BlueskyReddit
Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Invitrogen Antibodies

Product Name:

VCAM-1 Monoclonal Antibody

Catalog Number:

MA5-15636

VCAM-1 is a cell adhesion molecule that mediates the adhesion of lymphocytes, monocytes, eosinophils, and basophils to vascular endothelium. It also functions as leukocyte-endothelial cell signal transduction, and it may play a role in the development of atherosclerosis and rheumatoid arthritis. Here we checked the time course effect of chemoattractant on VCAM1 expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% BSA prepared in 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

There is no change in the VCAM1 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody is very good for western blot experiment

The Bad

None

The Bottom Line

This antibody works great for western blot with no background and non specific bands. Highly recommended.

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