MMP-9 Antibody

April 03, 2018

BlueskyReddit
Microbiology & Immunology
University of Rochester Medical Center
Research Scientist

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Company:

Cell Signaling Technology

Product Name:

MMP-9 Rabbit Ab

Catalog Number:

3852S

MMP-9 is a matrix metalloprotease produced from immune cells including macrophages and neutrophils, which plays important roles in wound healing, immunity, and angiogenesis. In this product review, I report results that the anti-mouse MMP-9 antibody from Cell signaling showed unsurpassed performance in detecting the protein in both Western blotting and immunofluroscence microscopy.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Neutrophil lysate from mouse

Primary Incubation

1:1000 dilution for 1 hr at room temp

Blocking Agent

2% BSA

Secondary Incubation

1:1000 HRP-conjugated anti-rabbit IgG (Cell signaling) 1 hr at room temp

Tertiary Incubation

None

Detection

Chemiluminescence (Pico from Pierce)

Results Summary

In the attached figure A, neutrophil total lysate (lane 3) and the supernatants from neutrophils stimulated with low (lane 1) and high (lane 2) amount of PMA were analyzed using Western blot analysis. Mouse MMP-9 protein was clearly detected with the expected size (between 90 and 100kDa). In the figure B, this antibody also successfully detected MMP-9 proteins in PFA-fixed neutrophils using immunofluorescence microscopy.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great performance even with half of the dose that the manufacturer suggests

The Bad

nothing

The Bottom Line

Cell signaling produces a lot of reliable antibodies, which are frequently cited in research articles. This antibody is one of them. I found this antibody worked well both in Western blotting and staining of fixed cells. One more good thing about antibodies from Cell signaling is that they are provided in a glycerol-containing buffer so that they are kept unfrozen at - 20C, which significantly lengthens their shelf-life.

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