Adipose Triglyceride Lipase Western Blot Antibody

March 30, 2018

Center for Translational Biomedical Research
UNC Greensboro
Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

Adipose Triglyceride Lipase antibody

Catalog Number:

2138S

This antibody was used in a study that investigated the role of lipin1 in ethanol induced adipose lipolysis. It has been published in Scientific Reports in 2018 (Zhang et al., Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury. 2018; 8: 408). The antibody reviewed here (2138S) gave best results in western blot with mouse white adipose tissue.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse white adipose tissue

Primary Incubation

1:1000, 10 hour at 4 degree

Blocking Agent

5% milk

Secondary Incubation

1:5000, 1 hour at 25 degree

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Ethanol feeding significantly increased the expression of ATGL.

DOI or PMID #

29323242

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to be detected by ECL

The Bad

Some non-specific bands

The Bottom Line

it is good for Western blot

Share your experience with other scientists. Write a Review! »

Join the discussion