Excellent Staining Of T Lymphocytes For CD25

Microbiology Immunology
University of Texas Medical Branch
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

CD25 PE/Dazzle 594

Catalog Number:

356125

The aim of the research is to determine activation markers for T lymphocytes and distinguish them for effector and memory cells. CD25 antibody was used for determining the change in population sets of these in patients with HIV at different stages of infection. Biolegend has exceptionally good Flow antibodies and great discounts so we ordered Cd25 from them and it worked great.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

30 minutes on ice

Blocking Agent

PBS with 5%BSA

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

I used 5ul per test as recommended and the antibody worked great.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Excellent antibody

The Bad

None

The Bottom Line

Works great!!!

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