Biolegend
FITC anti-mouse CX3CR1 Antibody
149020
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Browse Similar Products: CX3CR1 Flow Cytometry Antibodies
My work focuses on functions of CD8 T cells. From RNA-sequencing analysis, I had identified a CD44+ population of cells that expressed Cx3cr1, and chose this antibody to confirm the expression by flow cytometry. The top figure shown is an FMO control, and the bottom figure is staining of the Cx3cr1 antibody versus CD44. Both figures are gated on CD8 T cells. Flow cytometry data with this antibody confirmed the transcriptomics data, so this antibody seems to be a great tool for detecting Cx3cr1 protein expression. I would select this antibody again due to its low price and great results.
Flow Cytometry
Splenocytes from virus-infected mice
1:100 dilution in PBS with 2% FBS and 2 mM EDTA
2% FBS (in staining medium)
N/A
BD LSR II; 488 nm laser at 525 nm
Great staining of CX3CR1-expressing cells at a 1:100 dilution.
Great staining
Great antibody for detecting CX3CR1-expressing cells