Staining Mouse Splenocytes With Anti-CD45 APC/Cy7

March 28, 2018

BlueskyReddit
Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

anti-mouse CD45 APC/Cy7

Catalog Number:

103116

This antibody was used as a pan leukocyte marker in mouse splenocytes. It was chosen because it is a commonly used clone for CD45, APC/Cy7 is dim and therefore a good choice for a highly expressed protein, it works well with other fluorophores in the staining panel, and Biolegend had a competitive price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 dilution in 100uL total staining volume for 15 minutes at room temperature.

Blocking Agent

FcBlock + 2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

CD45 staining was as expected. Positive cells were fully resolved from negative.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well. Low cost

The Bad

N/A

The Bottom Line

Will continue to use this antibody.

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