Biolegend
FITC anti-mouse/rat CD29 Antibody
102206
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Browse Similar Products: CD29 Immunofluorescence Antibodies
Our lab is interested in the role of extracellular matrix and its receptors of the integrin family in controlling epithelial cell behaviour. Most of our assays are imaging based, therefore we wanted a readout to measure the efficiency of the beta1 KO in the cell lines we are using.
Immunofluorescence
NMuMG mammary epithelial cell line
1/200 dilution, 1hr at RT
5% BSA
None, it's a conjugated primary antibody
None
Confocal microscopy
The beta1 staining is strongly reduced in cell transfected with an siRNA targeting beta1, compared to a non-targeting siRNA control.
N/A
Very strong signal
Nothing
It worked very well in both 2D and 3D systems