Lyn Antibody from Cell Signaling Works Great

July 19, 2019

Biomedical Sciences
Cornell University
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Lyn (C13F9) Rabbit mAb #2796

Catalog Number:

2796

Our experiments aimed to understand the molecular mechanism of retinoic acid-induced differentiation. Various proteins of the putative signaling complex were investigated. This experiment determined endogenous levels of Lyn protein by Western blot in total lysate of Hl60 cells and primary human leukemic blasts using Lyn (C13F9) Rabbit mAb #2796 from Cell Signaling.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HL-60 cells; primary leukemia blasts

Primary Incubation

Overnight, 4C

Blocking Agent

BSA

Secondary Incubation

HRP anti-rabbit IgG, 1h, RT

Tertiary Incubation

None

Detection

chemiluminescence

Results Summary

Good antibody. High specificity and affinity.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good antibody. High specificity and affinity.

The Bad

None

The Bottom Line

Good antibody

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