Purified Mouse Anti-C-Raf Antibody

May 16, 2018

BlueskyReddit
Biomedical Sciences
Cornell University
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Purified Mouse Anti-c-Raf Clone 53/c-Raf-1 (RUO) BD Biosciences

Catalog Number:

610152

This product detects the c-Raf protein at the correct size ( 74 KDa) in total lysate, by Western blot. There is some background with lower molecular weight.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HL-60 cells; primary leukemia blasts

Primary Incubation

Overnight at 4 degrees celsius

Blocking Agent

BSA

Secondary Incubation

HRP anti-mouse, 1h, RT

Tertiary Incubation

None

Detection

Chemiluminescence

Results Summary

Good detection of the band of interest, however there is some background.

DOI or PMID #

10.18632/oncotarget.23642

Additional Notes

N/A

Related Categories

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Summary

The Good

Detects the band of interest

The Bad

Has background

The Bottom Line

It can be used for endogenous levels of c-Raf detection by Western blot.

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