PE HIV-1 Core Antigen Antibody

Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Beckman Coulter

Product Name:

HIV-1 CORE ANTIGEN-RD1

Catalog Number:

6604667

Our lab studies immune responses against HIV and we work with PBMC either from infected patients or healthy donor cells infected in vitro in order to study the role of different effector cells in killing HIV+ cells. We have a couple of antibodies in different colors from Beckam Coulter and they work well to detect intracellular p24 via FACS.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Latently HIV infected cells (ACH2) derived from humans activated with PMA

Primary Incubation

1:40 dilution in Perm/Fix Buffer for 1 hr at 4 degrees after fixing and permeabilization (using eBioscience FOXP3 staining kit)

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

Good results for a difficult staining

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Work great

The Bad

None

The Bottom Line

Good antibody for p24 detection

Share your experience with other scientists. Write a Review! »

Join the discussion