Anti-CD69 Antibody

Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC/Fire™ 750 anti-human CD69 Antibody

Catalog Number:

310946

We investigate ways to enhance the anti-HIV activity of NK cells. We activate the cells in vitro using different activators and then perform FACS analysis to examine the expression of different markers. We stain for CD69 as one of the activation markers.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human NK cells

Primary Incubation

1:20 dilution in PBS for 30 min in 4 degrees

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

Good antibody

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works well

The Bad

None

The Bottom Line

I recommend this antibody. I had to try a few to get the staining to work.

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