Anti-CD25 Antibody

Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-human CD25 Antibody

Catalog Number:

302606

We investigate ways to enhance the anti-HIV activity of NK cells. After using different activators we perform FACS analysis of the cells looking at the expression of different activator markers, CD25 being one of them.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human NK cells

Primary Incubation

1:20 dilution in PBS for 30 min in 4 degrees

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

Good detection of the activated cells. We can clearly see differences between different activators.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining

The Bad

None

The Bottom Line

Good antibody

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