Miltenyi Biotec
Neural Tissue Dissociation Kit (P)
130-092-628
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We use this kit to dissociate mouse brain tissue to isolate microglia for in vitro experiments. The kit provides an excellent way to dissociate brain tissue to isolate cells of interest.
Cell isolation/positive selection of cells
Mouse brain
Perfuse mouse with 1x PBS, harvest brain, follow kit instruction
Use the manual dissociation protocol if a machine is not available for dissociation
Single cell suspension of neural cell populations
N/A
Excellent dissociation for subsequent processing or positive selection methods
Can be somewhat time consuming
Good method for dissociating mouse brains for positive selection