Easy To Use, Accurate PCR Supermix

University of Colorado Denver
Medicine
Professional Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

Quanta Bio

Product Name:

AccuStart II GelTrack PCR SuperMix

Catalog Number:

95136

We work in mouse models and do a good deal of genotyping on our mice. We use the AccuStart II GelTrack PCR SuperMix in all of our PCRS for genotyping. It was recommended to us by another lab years ago and since then we have used it for all of our PCR needs - it is good quality, reasonably priced, and produces consistent results. It is room temperature stable and isn't affected by multiple freeze thaw cycles

Experimental Design and Results Summary

Application

PCR

Starting Material

Murine DNA

Protocol Overview

Simply add AccuStart to primers, water, and template for each reaction. Runs under a variety of PCR cycling conditions, making it easy to optimize for your amplicons. This Gel Track version already contains a loading buffer, so you can load your PCR samples directly into your gel.

Tips

Keep in mind that the Gel Track version comes with loading buffer in the master mix, but the regular AccuStart II does not.

Results Summary

Generates clear PCR products under a variety of cycling conditions. Works with a variety of starting materials.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to use, consistent, high-quality results

The Bad

Nothing - works great.

The Bottom Line

Great product. Would definitely recommend for PCR applications.

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