NK Cell Magnetic Bead Isolation Kit

Albert Einstein College of Medicine
Pathology
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD56+CD16+ NK Cell Isolation Kit, human

Catalog Number:

130-092-660

Our lab studies NK cell activity in the context of HIV infection and use purified NK cells from human PBMC. This kit provides reliable isolation of NK cells from this cell source.

Experimental Design and Results Summary

Application

In vitro NK cell experiments

Starting Material

Human PBMC

Protocol Overview

Isolate PBMCs, count cells, add beads, follow protocol according to product insert

Tips

If you want "unactivated" NK cells, skip the CD16+ positive selection step

Results Summary

Purified NK cells from PBMCs

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

High yield

The Bad

May have some stray non-NK cells in the mix

The Bottom Line

Cost-effective and time manageable method to purify human NK cells

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