Applied Biosystems
TaqMan™ Universal Master Mix II, no UNG
4440040
We use real-time qPCR to quantify murine cells transgenic for human genes in tissue our murine models. To do so, we sought a reliable mastermix for our TaqMan qPCR assays.
Real-time qPCR
Murine DNA
Extract DNA from tissue, amplify DNA according to manufacturers instructions
Be sure to UV sterilize your pipettes and consumables to reduce the chance of contamination
Reproducible results, consistent amplification of target, Ct values for standard curves consistently where they should be
N/A
Easy to use, straight forward protocols for designing and setting up and qPCR assay
Price
Highly recommended if seeking reliable, reproducible results for a long-running qPCR assay