Santa Cruz Biotechnology
LIMK-1 Antibody (H-12)
sc-515585
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Proteins containing LIM motifs are typically involved in cell fate determination and growth control. They are also involved in cell motility and migration. We determined the expression levels of LIMK-1 in Hela and SiHa Cells.
Western Blot
Cell Lysates
1:300 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% skimmed non-fat milk in PBST20
1:5000 anti-mouse HRP antibody, incubated for an hour at RT
None
ECL based detection was used
We found that this protein is expressed in both cell lines and this antibody gave the LIMK-1 specific bands in the Western Blotting.
N/A
Specificity and reactivity
Nothing
Overall, its a great antibody for Western Blotting.