Santa Cruz Biotechnology
PI 3-kinase p110δ Antibody (A-8):
sc-55589
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Phosphatidylinositol 3-kinase (PI 3-kinase) is a key protein that can activate AKt and several other proteins. It has a pivotal role in several cellular processes such as proliferation, apoptosis, and adhesion. We determined its expression levels in K562 cells before and after treatment with the drug of our interest.
Western Blot
Cell Lysates
1:1000 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% BSA in PBST20
1:5000 anti-mouse HRP antibody, incubated for an hour at RT
None
ECL based detection was used
This antibody worked well for western blotting purposes. Our treatment did not alter the expression levels of PI3K.
N/A
Specificity and reactivity
Nothing
It is a great antibody for Western blotting.