Santa Cruz Biotechnology
Atm Antibody (G-12)
sc-377293
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ATM is involved in DNA damage and repair pathway. Mutations in this gene are found in human cancers. We determined its expression levels in Hela and 293 T cells upon treatment with the drug of our interest.
Western Blot
Extracts of Hela and 293T
1:1000 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% skimmed non-fat milk in PBST20
1:10000 anti-mouse HRP antibody, incubated for an hour at RT
None
ECL based detection was used
We observed the single sharp band of ATM protein in our western blots without excessive background noise.
N/A
High specificity and instant development of blot
Nothing
Overall, its a great product.