Santa Cruz Biotechnology
p38α Antibody (9F12)
sc-81621
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p38 is a MAP kinase that has a diverse role in several cellular processes such as cell proliferation, adhesion, migration, and cytokine production. We performed Western blotting to probe p38aplha in Hela cells treated with different compounds. We determined the expression levels of p38 alpha after compound treatment.
Western Blot
Cell Lysates of Hela
1:1000 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% skimmed non-fat milk in PBST20
1:5000 anti-mouse HRP antibody, incubated for an hour at RT
None
ECL based detection was used
We observed the reduction in the expression levels of p38 upon the treatment with the compound of our interest.
N/A
Specificity and reactivity
Nothing
Overall, its a great antibody