Abcam
Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade
ab63766
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TATA binding protein has a significant role in the initiation of gene transcription and is often used a nuclear marker for Western blotting experiments. In our case, we used it as a loading control for Western blotting of nuclear lysates of Hela cells with different treatments.
Western Blot
Hela Nuclear Lysates
1:2000 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% skimmed non-fat milk in PBST20
1:10000 anti-rabbit HRP antibody, incubated for an hour at RT
None
ECL based detection was used
Overall, it is a great antibody that gives the right size band with a little bit of noise background.
N/A
Specificity and reactivity
Nothing
In conclusion, it is a good antibody