Santa Cruz Biotechnology
PCNA Antibody (PC10)
sc-56
Browse Similar Products: PCNA Western Blot Antibodies
Proliferating cell- Nuclear antigen is a 30 kDa protein that appears in early G and S phase of the cell cycle. It functions in DNA replication and is a nuclear marker for cell replication and often used as a loading control for western blotting.
Western Blot
Cell Lysates of NIH3T3
1:2000 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% skimmed non-fat milk in PBST20
1:10000 anti-mouse HRP antibody, incubated for an hour at RT
None
ECL based detection was used
We used this antibody as a loading control for our western blotting. This antibody worked really well and yielded a single band of PCNA.
N/A
Excellent antibody
In conclusion, it is one of the best PCNA antibodies for western blotting purposes.