Abcam
Anti-PCNA antibody
ab18197
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PCNA is a nuclear antigen that represents cellular proliferation. It is often used as a nuclear marker for Western blotting experiments especially those related to the cell cycle. We used 293T nuclear lysate to probe PCNA as a nuclear marker for loading control in western blotting.
Western Blot
Nuclear extracts of 293T
1:2000 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% skimmed non-fat milk in PBST20
1:5000 anti-rabbit HRP antibody, incubated for an hour at RT
None
ECL based detection was used
This antibody gave few non -specific bands along with the right band at approximately 30 kDa.
N/A
Good antibody
It gives a few non specific bands.
In total, it is a decent antibody.