Abcam
Anti-PCNA antibody [PC10] (ab29)
AB29
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PCNA is a nuclear antigen that is used as a proliferation marker for cell cycle-related experiments for western blotting. This protein is expressed in replicating cells in the nucleus. For our experiment, we prepared nuclear extracts of 293 T cells and probed with PCNA as a loading control.
Western Blot
Nuclear extracts of 293T
1:5000 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% skimmed non-fat milk in PBST20
1:10000 anti-mouse HRP antibody, incubated for an hour at RT
None
ECL based detection was used
anti-PCNA antibody from Abcam gave a single band at 30 kDa without any non-specific binding.
N/A
Excellent antibody
Nothing
Overall, its a great antibody for Western Blotting. The results are clean without any background noise.