Quanta Bio
PerfeCTa SYBR Green FastMix
95073-250
I am working on the development of molecular techniques for the detection of pathogens in the environmental samples. I have used this kit for amplification of bacterial DNA from environmental samples.
SYBR Green based qPCR amplification
Genomic DNA
Protocol is very easy to follow
Follow basic PCR guidelines and work with optimized annealing temperatures
Results obtained were good and reproducible
N/A
Easy to optimize and AccuFast hot start Taq DNA polymerase suppress primer elongation prior to the initial PCR denaturation step thus avoids non specific amplification
None
Good SYBR Green master mix and worth purchasing