qPCR Master Mix

Durban University of Technology
IWWT
Post doctoral Fellow
d

Overall

Quality of Results

Ease-of-Optimization

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Company:

Quanta Bio

Product Name:

PerfeCTa FastMix II ROX

Catalog Number:

95119-012

I am working on the development of molecular techniques for the detection of pathogens in the environmental samples. I have used this master mix for the qPCR amplification of bacterial/ viral DNA from environmental samples.

Experimental Design and Results Summary

Application

qPCR amplification

Starting Material

Genomic and plasmid DNA

Protocol Overview

Protocol is very much simple and easy to follow

Tips

optimize annealing temperature of your selected primers and sometimes initial denaturation temperature also needs optimization depending on the type and length of your template

Results Summary

Results obtained were good and consistent

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to work and light blue colur of AccuVue tracer dye helps to minimize pipetting errors in clear white plates and tubes

The Bad

None

The Bottom Line

Good performing master mix and worth purchasing

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