Quanta Bio
AccuStart II PCR ToughMix
95142-800
I am working in the field of environmental microbiology and my objectives include development of molecular methods for the detection of pathogens in environmental samples. I used this master mix for conventional PCR to detect virulence genes in the environmental water samples.
Conventional PCR
Genomic DNA
Protocol is quick and easy
Follow the instructions for initial denaturation settings but needs concentration optimization of different components of PCR reaction mix.
Overall this master mix works well for conventional PCR in detection of targated genes in environmental samples
N/A
Easy to optimize and repeated freeze and thaw doesn't effect its performance
None
Good master mix for conventional PCR with reproducible results.