Miltenyi Biotec
CD98-PE-Vio770, human (clone: REA387)
130-105-710
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The aim of this study was to observe activation markers upon CD3/CD28 stimulation and HIV infection of purified human CD4+ T cells. I selected this product as Miltenyi offers high quality antibodies at good prices, and is the only company offering CD98 Ab conjugated to PE-Cy7-like dye.
Flow Cytometry
Human PBMC (CD4+ isolated)
1:150 (50 ul), 30min, 4deg
NA
BD Fortessa-X20
We can observe good upregulation of CD98 expression after 3 days of CD3/CD28 stimulation and upon HIV infection compared to resting CD4+ T cells.
Good staining at high dilution
None
I would recommend this antibody for flow cytometry.