Good CD28 Staining On Human PBMCs

Infection and Immunity
University College London
PhD Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

APC-R700 Mouse Anti-Human CD28

Catalog Number:

565181

The aim of this study was to observe CD28 downregulation on CD4+ T cells infected with HIV encoding different Nef alleles. I chose this product as BD offers high quality antibodies at good price. Additionally, APC-R700 fluorochrome is brighter than Alexa700 and is offered exclusively by BD.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC (CD4+ isolated)

Primary Incubation

1:100 (50 ul), 30min, 4deg

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD Fortessa-X20

Results Summary

We can observe different levels of CD28 downregulation on CD4+ T cells infected with HIV encoding different Nef alleles.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Good staining at high dilution

The Bad

None

The Bottom Line

I would recommend this antibody for flow cytometry.

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