Excellent Glut-1 Staining On Human PBMCs

February 07, 2018

Infection and Immunity
University College London
PhD Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Glucose Transporter GLUT1 antibody [EPR3915] (Alexa Fluor® 647)

Catalog Number:

ab195020

The aim of this study was to observe upregulation of various activation markers upon CD3/CD28 stimulation of purified human CD4+ T cells. I selected this product as Abcam offers validated and high quality antibodies, but also because I was disappointed with Glut1 antibodies from other companies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

Surface antibodies, live/dead stain

Blocking Agent

NA

Secondary Incubation

Fix (4% PFA, 4deg, 20min) and Perm (100ul BD Perm buffer, 4deg, 20min)

Tertiary Incubation

Glut1 Ab: 1:600 (50ul Perm buffer), 4deg, 30min

Detection

BD Fortessa-X20

Results Summary

We can observe good upregulation of Glut1 transporter after 3 days of CD3/CD28 stimulation on CD3+CD4+ PBMCs .

DOI or PMID #

NA

Additional Notes

This Ab requires intracellular staining.

Related Categories

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Summary

The Good

Good staining at extremely high dilution

The Bad

None

The Bottom Line

I would recommend this antibody for flow cytometry.

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