Good HLA-DR Staining On Human PBMCs

February 21, 2018

BlueskyReddit
Infection and Immunity
University College London
PhD Student

Overall

Quality of Results

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Company:

Biolegend

Product Name:

Brilliant Violet 510™ anti-human HLA-DR Antibody

Catalog Number:

307645

The aim of this study was to observe activation markers upon CD3/CD28 stimulation of purified human CD4+ T cells. I selected this product as Biolegend offers high quality antibodies at good prices.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC (CD4+ isolated)

Primary Incubation

1:100 (50 ul), 30min, 4deg

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD Fortessa-X20

Results Summary

We can observe good upregulation of HLA-DR, which correlates with CD38 expression after 3 days of CD3/CD28 stimulation.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Good staining at high dilution

The Bad

None

The Bottom Line

I would recommend this antibody for flow cytometry.

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