Reliable CD14 Antibody

February 28, 2018

BlueskyReddit
Cell Biology
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Invitrogen Antibodies

Product Name:

CD14 Monoclonal Antibody (TuK4), TRI-COLOR

Catalog Number:

MHCD1406

The general aim of the research is to study hematopoiesis and acute myeloid leukemia. This antibody was to measure the effect of differentiation agents on the leukemic cell line THP-1.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

THP-1 cell line

Primary Incubation

1:50 dilution for 30 minutes on ice

Blocking Agent

Human Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on a BD Aria unit

Results Summary

This antibody detected increased expression of CD14 in THP-1 cells treated with a differentiation agent (blue peak).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Gives a very strong signal

The Bad

None

The Bottom Line

Recommended for CD14 detection on human blood cells

Share your experience with other scientists. Write a Review! »

Join the discussion