Strong CD40 Signal

March 08, 2018

BlueskyReddit
Cell Biology
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-human CD40 Antibody

Catalog Number:

334307

The general aim of the research is to study hematopoiesis and acute myeloid leukemia. This antibody was to measure the effect of differentiation agents on the leukemic cell line THP-1.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

THP-1 cell line

Primary Incubation

1:50 dilution for 30 minutes on ice

Blocking Agent

Human Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on a BD LSRII unit

Results Summary

Increased expression of CD40 was detected after treatment of THP-1 cells with a differentiation agent (blue peak)

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong signal

The Bad

None

The Bottom Line

I recommend this antibody for CD40 surface expression detection

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