Mouse CD11c PerCP-Cy5.5 For FACS

March 02, 2018

Microbiology & Immunology
Columbia University
MD/PhD Student

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Company:

Biolegend

Product Name:

PerCP/Cy5.5 anti-mouse CD11c Antibody

Catalog Number:

117327

Dendritic cells (DCs) mediate adaptive immune responses through the presentation of antigens to lymphocytes. DCs are heterogeneous and are found in different anatomical sites in both mice and humans. Expression of the integrin CD11c marks mature DCs and can be used to identify these populations by flow cytometry. We are interested in the development of mouse dendritic cells in vitro and in vivo and how metabolic signals may regulate this process. The mouse CD11c PerCP-Cy5.5 antibody was selected for multiplexing with a 10-color flow cytometry panel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:400; 20 minutes, 4C

Blocking Agent

2% FBS in dPBS 2mM EDTA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD Fortessa

Results Summary

Spleens from C57BL/6 mice were harvested and homogenized to obtain a single cell suspension. Following red blood cell lysis, samples were stained to detect mature dendritic cell subsets. DCs were identified as CD11c+ MHCII+. A clear DC population was detectable and could be further analyzed for subsets specific markers. Staining and separation was maintained after aldehyde fixation/permeablization.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy detection of infrequent DC population at low dilutions; reasonably priced

The Bad

none

The Bottom Line

The mouse CD11c PerCP-Cy5.5 antibody is an excellent choice for detecting dendritic cells across organs and can be multiplexed for identification of multiple sub-lineages.

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