Biolegend
Biotin anti-mouse CD197 (CCR7) Antibody
120103
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Browse Similar Products: CD197 Flow Cytometry Antibodies
The aim was to study T cell trafficking. The specific goal was to examine the expression of CCR7 chemokine receptor (homing to secondary lymphoid organs) by CD4 T cells. I selected this product because it was of high quality and is cost effective.
Flow Cytometry
Mouse splenocytes
1:20 (2ul of antibody in 40ul of sample)
5% rat serum
1:1000 (Streptavidin-APC)
NA
APC
Works well for flow cytometry
High quality with low cost
This 2 step staining with biotin-antibody is better than one-step staining with fluorochrome-conjugated antibody