Biolegend
BV605 anti-Human CD45RA
304134
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Browse Similar Products: CD45RA Flow Cytometry Antibodies
One of the main aims in our lab is to study T cell activity in humans. In order to determine between different T cell populations in various human tissue samples, we can use this antibody to segregate T cells into memory and non-memory populations.
Flow Cytometry
Human spleen cells
Cells were stained in 100uL FACS buffer with antibody at 1:200; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS buffer
None
LSR II
We successfully stained human CD45RA+ cells isolated from the spleen.
N/A
Good brightness
This antibody is not too bright as to create spillover, but is bright enough to clearly identify positive populations.