Biolegend
APC/Cy7 anti-mouse/human CD44 Antibody
103028
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In our lab, we are interested in studying how T cells not only function, but also change before, during, and after an influenza A viral infection. One of the pillars for this study is being able to identify and separate the different T cells populations for downstream analysis. This marker is good for CD44 because it is not to bright to overwhelm the smaller populations.
Flow Cytometry
Mouse lung cells
Cells were stained in 100uL FACS buffer with antibody at 1:200; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS buffer
None
LSR II
We easily stained the large CD44+ populations.
N/A
Dim for large population sizes
This antibody allowed us to quickly identify our CD44 positive and negative cell fractions for further analysis.