MHC II APC for BMDCs

February 23, 2018

Immunology
The Jackson Laboratory for Genomic Medicine
Lab Manager, RA III

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

APC anti-mouse I-A/I-E Antibody

Catalog Number:

107613

This antibody was used to stain BMDC for MHC II and other surface molecules to make sure they differentiated correctly in culture during a survival assay.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse BMDC

Primary Incubation

1/50 dilution in PBS+ 2%FBS for 15min at RT at dark.

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSR II

Results Summary

Good separation of Dendritic cells MHCII +

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright, nice separation

The Bad

N/A

The Bottom Line

good antibody

Share your experience with other scientists. Write a Review! »

Join the discussion